Merck, SLM-240-B, RPMI 1640 Complete Medium, with 2mM L-Glut and 10% FBS

Name: RPMI 1640 Complete Medium, with 2mM L-Glut and 10% FBS
Brand: Merck
SKU: SLM-240-B

Size:500 mL, Low Bicarb Formulation, Roswell Park Memorial Institute 1640 medium, supplemented with 2mM L-Glutamine and 10% U.S. origin FBS

Catalog Number: SLM-240-B

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Category

Classical Cell Culture Media

Pack Size500 mL

TagsCell Culture & Analysis Cell Culture Media & Buffers Classical Media & Buffers

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
RPMI 1640 CompleteMedium is supplemented with 2mM L-Glutamine and 10% U.S.-origin fetal bovine serum (FBS). This solution is convenient to culture a wide range ofcells that grow in suspension or as anchorage dependent. It can also be used infusion protocols and for growing hybrid cells. Roswell Park Memorial Institute(RPMI) 1640 medium can be used for culturing human normal and neoplasticleukocytes. It supports the growth of various cultured cells, like fresh humanlymphocytes in the 72-hour phytohemagglutinin (PHA) stimulation assay if theformulation is properly supplemented.
Application
RPMI 1640 CompleteMedium, with 2mM L-Glut and 10% FBS has been used to culture: Jurkatlymphocytes and human U937 monocyte-like cell line(1), HT-29 and SW480 (humancolon cancer cell line) cells(2), monocytes(3)
Analysis Note
pH= 7.2-7.5
Osmolality= 270-292 mOsm/kg
Endotoxin= ≤2 EU/mL
Mycoplasma= Negative
Sterility Tested= Negative for Growth
Appearance= Orange/Red Clear Liquid
Cell Line Tested= A2780 Human Ovarian Carcinoma

Specifications

General

UNSPSC Code	12352207
eCl@ss	32160801
Web Name Override	RPMI-1640 complete medium
NACRES	NA.71
Web Name Suffix Override	With ʟ-glutamine and 10% US origin FBS, liquid, 0.2 μm filtered, suitable for cell culture
form	liquid
technique(s)	cell culture \| mammalian: suitable
Quality Level	300

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