Merck, ST1507-100UG, Anti-AKR1B10 Mouse mAb (1A6)

General description
Anti-AKR1B10. Mpuse monoclonal, clone 1A6 recognizes the ~37 kDa AKR1B10 protein in HepG2 and HeLa cells and human colon tissue. It is validated for ELISA, WB, ICC, and paraffin sections.
Purified mouse monoclonal antibody. Recognizes the ~37 kDa AKR1B10 protein.
Recognizes the ~37 kDa AKR1B10 protein in HepG2 and HeLa cells and human colon tissue.
Immunogen
A recombinant polypeptide corresponding to amino acids 76-144 of human AKR1B10, expressed as a GST fusion protein
NA
Application

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (3 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
HepG2 cells, Human Colon tissue, HeLa cells
Other Notes
Ahn K.H., et al. 2010 JOURNAL OF HEALTH SCIENCE56, 287.
Satow, R., et al. 2010. Clin. Cancer Res.16, 2518.
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)