Merck, ST1517-100UG, Anti-PIP5K3 Mouse mAb (6C7)

General description
Purified mouse monoclonal antibody. Recognizes the ~60 kDa PIP5K3 protein.
Recognizes the ~60 kDa PIP5K3 protein in human testes tissue and HeLa cells.
This Anti-PIP5K3 Mouse mAb (6C7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of PIP5K3.
Immunogen
A recombinant polypeptide corresponding to amino acids 342-452 of human PIP5K3, expressed as a GST fusion protein
Human
Application

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (3 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Negative Control
293T cells
Positive Control
Human testes tissue, HeLa cells
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Tsuruta, F., et al. 2009. J. Cell Biol.187 279.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)