Merck, ST1533-100UG, Anti-ALDH2 Mouse mAb (1E5)

General description
Anti-ALDH2, mouse monoclonal, clone 1E5, recognizes the ~50 kDa ALDH2 protein in MCF-7 cells. It is validated for ELISA and Western blotting.
Purified mouse monoclonal antibody. Recognizes the ~50 kDa ALDH2 protein.
Recognizes the ~50 kDa ALDH2 protein in MCF7 cells.
Immunogen
A recombinant polypeptide corresponding to amino acids 408-517 of human ALDH2, expressed as a GST fusion protein
Application

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
MCF7 cells
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 µg/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)