Merck, ST1641-100UG, Anti-MAP4K4 Mouse mAb (4A5)

General description
Purified mouse monoclonal antibody. Recognizes the ~140 kDa MAP4K4 protein.
Recognizes the ~140 kDa MAP4K4 protein in K-562 cells and human breast cancer tissue.
This Anti-MAP4K4 Mouse mAb (4A5) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of MAP4K4.
Immunogen
A recombinant polypeptide corresponding to amino acids of 611-710 human MAP4K4, expressed as a GST fusion protein
Application

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Paraffin Sections (3 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
K-562 cells, Human breast cancer tissue
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)