Merck, ST1705-100UG, Anti-TOMM20 Mouse mAb (4F3)

General description
Purified mouse monoclonal antibody. Recognizes the ~12-16 kDa TOMM20 protein.
Recognizes the ~12-16 kDa TOMM20 protein in HeLa, PC12, and NIH3T3 cells and human small intestine tissue.
This Anti-TOMM20 Mouse mAb (4F3) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of TOMM20.
Immunogen
Full-length, recombinant, human TOMM20 (aa 1-146), expressed as a GST fusion protein
Application

ELISA (see coments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (3 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Negative Control
293T cells
Positive Control
HeLa cells, PC12 cells, NIH3T3 cells, Human small intestine tissue
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)