Merck, TRUE1-1KT, TrueGel3D Hydrogel Kits

Name: TrueGel3D Hydrogel Kits
Brand: Merck
SKU: TRUE1-1KT

Size:1 kit, CD cell-degradable crosslinker and RGD peptide

Catalog Number: TRUE1-1KT

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Category

Hydrogels & Matrices

Tags3D cell culture Cell Culture & Analysis Hydrogels

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
The TrueGel3D hydrogel pre-configured kit is used to prepare a chemically defined hydrogel with stiffness of ~400 Pa (shear modulus) that can be customized to match that of native cell environment. The TrueGel3D hydrogel is formed by crosslinking of RGD (arginylglycylaspartic acid) degradable polymer with CD cell-degradable crosslinker. The cells are encapsulated during crosslinking, where they can adhere to the polymer through the RGD peptide and grow within the hydrogel. The CD cell-degradable crosslinker is composed of matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala), which allows cells to spread and migrate by secreting matrix metalloproteases (MMP1, MMP3, MMP7 and MMP9).

Gel can be further dissolved by TrueGel3D Enzymatic Cell Recovery Solution (TRUEENZ-500UL) for post-culture analysis including RT-PCR, western blots and other molecular biological studies.
Application
Suitable for 3D-culture of various cell types, spheroids and tissue samples. It has been used for cell migration and invasion studies, co-culture experiments (tumor cells with fibroblasts) and cell growth and differentiation studies.
Features and Benefits

Easy to use and pre-configured
Gel with soft stiffness of 400 Pa
Contains RGD degradable polymer for cell attachment
Allow cell recovery and post-culture analyses

Other Notes
TRUE1-1KT contains lyophilized RGD degradable polymer and CD cell-degradable crosslinker with reconstitution buffer and water.
Disclaimer
Avoid prolong exposure of CD cell-degradable crosslinker to air.

Specifications

General

NACRES	NA.71
UNSPSC Code	12352207
shipped in	dry ice
storage temp.	−70°C

FAQs

Question: What are the key kit components?

Answer:RGD-functionalized degradable polymer, cell-degradable crosslinker, reconstitution buffer, and water.

Question: What is the approximate gel stiffness?

Answer:The prepared gel has a shear modulus of approximately 400 Pa under specified conditions.

Question: How is the kit stored?

Answer:The kit is shipped on dry ice and stored at −70 °C.

Application Note

TrueGel3D™ is a preconfigured chemically defined hydrogel system for three-dimensional culture of cells, spheroids, and tissue samples. This kit combines lyophilized RGD-functionalized degradable polymer with a cell-degradable crosslinker, reconstitution buffer, and water. The RGD peptide provides cell-attachment cues, while matrix-metalloprotease-sensitive crosslinks allow encapsulated cells to remodel their surroundings, spread, and migrate. Under the specified preparation conditions, the resulting gel has a soft shear modulus of approximately 400 Pa, making it useful for studies of cell migration, invasion, growth, differentiation, and co-culture. The hydrogel can be enzymatically dissolved with the separately available recovery solution to enable downstream analysis. This formulation ships on dry ice and has a storage specification of −70 °C.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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