Description
General description
The gel is formed by stable thioether bonds between maleimide groups of dextran (FAST-DEXTRAN) and thiol groups on CD cell-degradable crosslinker. Malemide-functionalized dextran is specifically used to decrease the time for gel formation. The dextran polymer does not have cell attachment sites, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1UM) prior to the crosslinking step. The CD cell-degradable crosslinker is composed of matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala), which allows cells to spread and migrate by secreting matrix metalloproteases (MMP1, MMP3, MMP7 and MMP9).
TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.
TrueGel3D® provides a high degree of control in a 3D hydrogel environment, providing greater consistency. It enables to fine-tune the microenvironments around the tissues or cells under study.
Application
TRUE3-1KT is used for cell migration studies of amoeboid cells including neutrophils, dendritic cells and lymphocytes. It has been used to study the effect of mechanic stimulus over tumor development, tumor progression and cell differentiation.
Features and Benefits
Fast gelling (takes few seconds to minutes after addition of crosslinker)
Contains MMP-cleavable crosslinker
Allow cell recovery and post-culture analyses
Flexibility in modulating gel stiffness
Cells do not attach to hydrogel (if not customized with TrueGel3D RGD peptide)
Other Notes
TRUE3-1KT contains lyophilized FAST-DEXTRAN (polymer) and CD cell-degradable crosslinker with reconstitution buffer, TrueGel3D thioglycerol and water.
Legal Information
TRUEGEL3D is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Avoid prolonged exposure of CD cell-degradable crosslinker to air.
