Merck, UFC803096, Amicon® Ultra Centrifugal Filter, 30 kDa MWCO

Name: Amicon® Ultra Centrifugal Filter, 30 kDa MWCO
Brand: Merck
SKU: UFC803096

Size:96 pkg, Centrifugal concentrator, 30 kDa MWCO, 4 mL sample volume, sample volume 4 mL, regenerated cellulose membrane, MWCO 30 kDa

Catalog Number: UFC803096

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Category

Ultrafiltration Spin Columns

TagsFiltration Concentration Filters Amicon<sup>®</sup> Centrifugals

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Amicon® Ultra 4 mL centrifugal devices with Ultracel® 30,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, Ovalbumin (45 kDa) at 1 mg/ml initial concentration is retained at >90% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.

The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from 60% bio-circular material allocated using the ISCC mass balance approach. Click here for more information.
Application
Ultrafiltration with 30,000 Dalton molecular weight cutoff Ultracel® membrane.
Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC.
Concentration of dilute or pre-purified proteins from column eluents.
Desalting, buffer exchange and protein dialysis.
Size exclusion and protein fractionation.

Features and Benefits
Ultracel® regenerated cellulose membrane with 30 kDa NMWCO delivers retentate recovery of greater than 90%.
Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes).
Heat-sealed membrane minimizes downstream extractables.
100% integrity tested for reliable performance.
Convenient sample monitoring with translucent housing and volume gradations.
Direct pipette sample access eliminates processing step to recover concentrate.
High concentration factors of 80–100X.

Physical form
Color Code: Clear with pink cap
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Specifications

General

UNSPSC Code	41104916
NACRES	NB.24
eCl@ss	36100101
material	polyethylene cap
sterility	non-sterile
product line	Amicon®
packaging	pkg of 24 pkg
manufacturer/tradename	Amicon® Ultra
sustainability	Greener Alternative Product
parameter	4 mL sample volume
technique(s)	protein extraction: suitable
L	124 mm (4.88 in.)
diam.	17.3 mm
filtration area	3 cm2
volume	4 mL
working volume	≤4 mL
pore size	30 kDa MWCO
Quality Level	400
greener alternative category	Re-engineered
shipped in	ambient

FAQs

Question: What is the molecular-weight cutoff?

Answer:The membrane has a 30 kDa MWCO.

Question: What sample volume can each device process?

Answer:The Ultra-4 format accepts up to 4 mL.

Question: How many filters are included?

Answer:This configuration contains 96 centrifugal filter devices.

Application Note

Amicon® Ultra-4 centrifugal filters are ultrafiltration devices for concentrating, desalting, and buffer exchanging macromolecular samples. This configuration uses an Ultracel® regenerated-cellulose membrane with a 30 kDa molecular-weight cutoff, accepts up to 4 mL of sample, and is supplied as 96 devices. The vertical membrane design provides approximately 3 cm² of filtration area and helps reduce concentration polarization during centrifugation. The device can achieve high sample recovery and concentration factors while a built-in dead-stop design helps prevent spinning the sample completely dry. Typical applications include concentration of proteins, antibodies, enzymes, nucleic acids, and microorganisms, as well as buffer exchange and removal of smaller solutes. The devices are supplied non-sterile.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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