Merck, UPBSTRT-1KT, Ultra Pure BST-RT Kit

General description
BST-RT Polymerase is an engineered Bst enzyme with robustreverse transcriptase (RT) activity, making it ideal for use in loop-mediatedisothermal amplification (LAMP) or RT-LAMP assays. This feature eliminates thenecessity of a secondary RT enzyme when targeting amplification from RNAsamples, simplifying reaction setup. BST-RT works optimally at 65°C and can beused for real time reaction monitoring with a fluorescent dye or in endpointdetection assays. BST-RT polymerase is tolerant to potential reactioninhibitors such as ethanol, extraction buffers, and saliva. This enzyme is alsocompatible with workflows that incorporate dUTP for elimination of reactioncarryover contaminants.
Application
Loop-mediated isothermal amplification (LAMP) has emerged asa key alternative to polymerase chain reaction (PCR)-based techniques foramplifying nucleic acids. While conventional PCR employs a thermal cycler forrepetitive cycling temperatures when amplifying, isothermal amplificationtechniques occur at a single and fixed temperature, allowing the use of simple,portable, and more robust instruments for fast and exponential amplification.
Features and Benefits

• Compatible with samples containing either DNA orRNA
• Ability to perform RT-LAMP without additional RTenzymes
• Lacks 5’ to 3’ exonuclease domain
• DNase free, RNase free, DNA free, and Nickasefree
• Active from 25-65 °C (optimal at 65 °C)
• Unit Definition: One unit is the amount ofenzyme required to incorporate 10 nmol of dNTP onto ssDNA template for 30minutes at 65°C.
• Also available in stand-alone, highconcentrations and glycerol-free formulations