Miltenyi, 130-046-701, Indirect CD34 MicroBead Kit, human

Background information
CD34 is a well-established marker of human hematopoietic stem and progenitor cells and additionally expressed on hemangioblasts1,2, endothelial progenitor cells1,3-5, and mature endothelial cells4.

Detailed separation procedure
First, cells are labeled with the hapten-modified CD34 primary antibody QBEND/106. In a second step, antibody-labeled cells are magnetically labeled with Anti-Hapten MicroBeads.
Control staining of cells isolated with the Indirect CD34 MicroBead Kit requires a monoclonal CD34 antibody other than QBEND/10, e.g. clone AC136

Downstream applications

- Transplantation of CD34+ cells into humanized mouse models
- Expansion and differentiation in culture
- Reprogramming of CD34+ HSCs into iPS cells
- Differentiation of ES or iPS cell-derived CD34+ -derived precursors into endothelial or hematopoietic lineages
- Evaluation of differentiation potential in CFU assays
- Characterization of surface marker expression by flow cytometry.

Columns
MS, LS, XS, or autoMACS® Columns.

Overview
The Indirect CD34 MicroBead Kit was developed for the isolation of CD34+ precursor cells, using a two-step procedure involving indirect magnetic labeling from
- peripheral blood
- bone marrow
- leukapheresis product
- cord blood
- differentiated-ES and iPS cells
Even faster, one-step isolation of CD34+ cells can be achieved with the CD34 MicroBead Kit.