Miltenyi, 130-046-703, CD34 MicroBead Kit, human

Background information
CD34 is a well-established marker of human hematopoietic stem and progenitor cells and additionally expressed on hemangioblasts5,6, endothelial progenitor cells5,7-9, and mature endothelial cells8.
Control staining of cells isolated with the CD34 MicroBead Kit requires a monoclonal CD34 antibody other than QBEND/10, e.g., clone AC136.

Downstream applications
Cells isolated with the CD34 MicroBead Kit are suitable for various downstream applications, including:
- flow cytometric analysis
- expansion in culture
- in vitro differentiation5
- reprogramming3
- retroviral transduction10
- gene expression analyses
- transplantation into humanized mice

Columns
MS, LS, XS, or autoMACS® Columns.

Overview
The CD34 MicroBead Kit has been developed for the positive selection of CD34+ hematopoietic stem and progenitor cells in a single step. CD34+ cells can be isolated from:
- peripheral blood (PBMCs)
- bone marrow1
- leukapheresis product2
- cord blood3
- differentiated ES and iPS cell lines4,5.