Miltenyi, 130-048-701, Anti-FITC MicroBeads

Background information
The FITC molecule is recognized by the monoclonal anti-FITC antibody coupled to MicroBeads, allowing the positive selection or depletion of the fluorescently labeled material with MACS® Technology. Labeling samples with FITC-conjugated primary antibodies and Anti-FITC MicroBeads enables subsequent flow cytometric analysis of separated cells without any further staining of the material.

Applications
Examples among the many separations performed with Anti-FITC MicroBeads are the isolation of IgD+ B cells from human PBMCs1, the separation of plant protoplasts2 as well as the isolation of murine Langerhans cells from epidermal tissue3. Anti-FITC MicroBeads have also been used for the depletion of CD3+ cells from rat spleen and subsequent isolation of CD3- NK cells5 or the separation of murine naive CD8+ T cells expressing low levels of CD44 by depletion of CD44int/high cells6.

Columns
For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, D, or autoMACS Columns.

Overview
Anti-FITC MicroBeads are widely used for indirect magnetic labeling and separation of cells or other materials which are labeled with a primary antibody or ligand conjugated to fluorescein isothiocyanate (FITC).