Miltenyi, 130-049-301, CD5 (Ly-1) MicroBeads, mouse

Background information
The CD5 antigen is highly expressed on T cells. CD5 is also expressed on B-1a cells but not on conventional B-2 cells. B-1 cells differ from B-2 cells with regard to origin, development, and tissue distribution and show an antigen-restricted B cell receptor repertoire.

Applications
CD5 MicroBeads can also be used for the positive selection of T cells and subsequent analysis of cytokine secretion kinetics during T cell differentiation.
For the isolation of highly pure B-1a cells by positive selection using CD5 MicroBeads, T cells have to be depleted in advance by using CD90.2 MicroBeads.

Columns
For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, D, or autoMACS Columns.

Overview
Mouse CD5 (Ly-1) MicroBeads can be used for the positive selection or depletion of T cells or CD5+ B-1a cells from peripheral lymphoid organs, non-lymphoid tissue or blood.