Miltenyi, 130-056-701, CD34 MultiSort Kit, human

Detailed separation procedure
Using the CD34 MultiSort Kit, in the first step CD34+ cells are positively selected after labeling with the CD34 MultiSort MicroBeads. Subsequently, the MultiSort MicroBeads are enzymatically released from the CD34 antibody. The isolated CD34+ cells can be magnetically labeled and sorted according to a second antigen. The kit contains CD34 MultiSort MicroBeads, MultiSort Release Reagent, and MultiSort Stop Reagent.
Control staining after separation requires a monoclonal CD34 antibody other than QBEND/10, e.g. clone AC136

Downstream applications
The CD34 MultiSort Kit is used to isolate specific subsets of CD34+ hematopoietic stem and progenitor cells. CD34-selected cells can be further sorted according to their expression of, e.g., HLA-DR, CD43, CD45RA, CD61, CD71, CD90 (Thy1), or CD133.

Columns
MS, LS, XS, or autoMACS® Columns.

Overview
The CD34 MultiSort Kit has been developed for the positive selection of CD34+ subpopulations by sequential sorting. CD34+ hematopoietic stem and progenitor cells can be isolated from
- peripheral blood,
- bone marrow aspirate,
- leukapheresis product,
- cord blood,
- differentiated ES and iPS cell cultures1.