Miltenyi, 130-061-101, CD326 (EpCAM) MicroBeads, human

Background information
CD326, also known as human epithelial antigen (HEA), epithelial cell adhesion molecule (EpCAM), or epithelial-specific antigen (ESA) expression is seen in the majority of carcinomas. It is expressed on cells of epithelial origin, epithelium-derived tumor cells7, circulating tumor cells, and cancer stem cells.

Downstream applications
Tumor cells enriched by CD326 (EpCAM) MicroBeads can be cultured8 and analyzed by immunocytochemistry9, flow cytometry, or molecular biology techniques, for example, RT-PCR10.

Columns
MS, LS, XS, or autoMACS® Columns.

Overview
CD326 (EpCAM) MicroBeads can be used for the:
- positive selection or depletion of epithelial cells or epithelial tumor cells from single-cell preparations of tissues and organs,
- enrichment of circulating or disseminated epithelial tumor cells expressing CD326 from peripheral blood, bone marrow, or even lymph nodes, serous effusions, or stool samples1 of patients with carcinomas,
- positive selection of functional pluripotent hepatic progenitor cells (hepatic stem cells and hepatoblasts) from fetal, neonatal, pediatric, and adult liver tissue2,3,
- positive selection of tumor exosomes from peripheral blood4,
- enrichment of exfoliated epithelial cells from gastric aspirates and stool samples of preterm infants5,
- purification of primary tumor cell cultures6.