Miltenyi, 130-090-452, Anti-Melanoma (MCSP) MicroBeads, human

Background information
The antibody clone 9.2.27 recognizes the melanoma-associated chondroitin sulfate proteoglycan (MCSP) antigen, also known as high molecular weight melanoma-associated antigen, or NG2. MCSP is expressed on melanoma cells but not on carcinoma cells, fibroblastoid cells, or cells of hematopoietic origin.

Downstream applications
Cells enriched with Anti-Melanoma (MCSP) MicroBeads can be analyzed by immunocytochemistry, flow cytometry, or molecular biology methods. The isolated cells can also be cultured.

Columns
MS, LS, or autoMACS® Columns.

Overview
Anti-Melanoma (MCSP) MicroBeads were developed for
- enrichment of disseminated melanoma cells from peripheral blood1, bone marrow, leukapheresis harvest, and lymphoid tissue of melanoma patients – even in early disease stages2,3,
- isolation of melanoma cells from single-cell suspensions of primary tumor tissue or primary skin cell culture.
Anti-Melanoma MicroBeads can be used in combination with the Inside Stain Kit. This enables the in-column staining of enriched melanoma cells for intracellular antigens, e. g., Melan-A. By performing combined enrichment and staining, a sensitivity of melanoma cell detection of one melanoma cell in 2×107 PBMC background cells can be achieved4. For convenient subsequent immunocytochemical detection the Melanoma Enrichment and Detection Kit can be used.