Miltenyi, 130-090-757, Anti-PE MultiSort Kit

Detailed separation procedure
Cells are first stained with a MACS® PE-conjugated primary antibody and subsequently magnetically labeled with Anti-PE MultiSort MicroBeads. Following positive selection, the magnetic particles are removed from the cells by using the MultiSort Release Reagent. The cell population can then be magnetically labeled and sorted using a second marker of interest. This is achieved by labeling the cells directly or indirectly with MACS® MicroBeads.

Applications
The Anti-PE MultiSort Kit is recommended if a positive selection according to two cell surface antigens is desired. The kit can, for example, be used for the positive selection of rodent T cell, B cell, or progenitor cell subsets.

Columns
MS, LS, XS, or autoMACS® Columns.

Overview
The Anti-Phycoerythrin (PE) MultiSort Kit provides a flexible tool for the sorting of cells according to multiple surface markers. The bright fluorescence intensity of PE-conjugated antibodies makes the Anti-PE MultiSort Kit especially useful for separation and subsequent detection of cells with weakly expressed markers.