Miltenyi, 130-090-862, B Cell Isolation Kit, mouse

Detailed separation procedure
Highly pure resting B cells are isolated by magnetic labeling and depletion of CD43-expressing B cells (activated B cells, plasma cells and CD5+ B-1a cells) and non-B cells. CD43+ B cells, T cells, NK cells, dendritic cells, macrophages, granulocytes, and erythroid cells are labeled with a cocktail of biotin-conjugated antibodies against CD43 (Ly-48), CD4 (L3T4), and Ter-119, as well as Anti-Biotin MicroBeads. The B Cell Isolation Kit includes the Biotin-Antibody Cocktail for labeling of non-target cells and Anti-Biotin MicroBeads.

Downstream applications
Untouched B cells may be used for further magnetic separation of B cells into subpopulations. B cells isolated with the B Cell Isolation Kit were e.g. analyzed for transgene expression by Northern blot analysis in a transgenic reporter model.1

Columns
MS, LS, or autoMACS® Columns.

Overview
The B Cell Isolation Kit was developed for the fast and efficient isolation untouched resting B cells from single-cell suspensions of lymphoid tissues. The separation protocol is faster now! Download the updated datasheet.