Miltenyi, 130-090-874, StraightFrom® Whole Blood CD3 MicroBeads, human

Background information
StraightFrom® Whole Blood CD3 MicroBeads recognize the CD3 antigen which is associated with the T cell receptor (TCR) heterodimer. CD3 is expressed on all T cells, constituting 15–20% of human peripheral blood leukocytes. StraightFrom Whole Blood MicroBeads allow the fast isolation of CD3+ cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yield of target cells. StraightFrom Whole Blood MicroBeads in combination with the autoMACS® Pro Separator allow standardization of the cell separation procedure and safe handling of hazardous samples.

Detailed separation procedure
Anticoagulated cell samples of 0.25–15 mL are labeled with StraightFrom® Whole Blood CD3 MicroBeads to be subsequently separated with the autoMACS® Pro Separator, the MultiMACS™ Cell24 Separator Plus, or using the Whole Blood Column Kit.

Downstream applications
The purified CD3+ cells are well-suited for further flow cytometric, functional, or molecular analysis including lineage-specific chimerism analysis after allogeneic stem cell transplantation1,2.

Columns
autoMACS® Columns or Whole Blood Columns.

Overview
StraightFrom® Whole Blood CD3 MicroBeads were developed for rapid positive selection of CD3+ cells directly from whole blood or bone marrow. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis.