Miltenyi, 130-090-877, StraightFrom® Whole Blood CD4 MicroBeads, human

Background information
StraightFrom® Whole Blood CD4 MicroBeads recognize about 65% of all peripheral blood T cells. The CD4 antigen is expressed on T helper cells and at a lower level on monocytes and dendritic cells. The CD4 antigen is an accessory molecule in the recognition of MHC class II/peptide complexes by the TCR heterodimer on CD4+ T helper cells.

Detailed separation procedure
Anticoagulated cell samples of 0.25–15 mL are labeled with StraightFrom® Whole Blood CD4 MicroBeads to be subsequently separated with the autoMACS® Pro Separator, the MultiMACS™ Cell24 Separator Plus, or using the Whole Blood Column Kit.

Downstream applications
StraightFrom® Whole Blood MicroBeads allow the fast isolation of CD4+ cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yields of target cells. StraightFrom Whole Blood MicroBeads in combination with the autoMACS® Pro Separator allow standardization of the cell separation procedure and safe handling of hazardous samples. The purified CD4+ cells are well-suited for further flow cytometric, functional, or molecular analysis including lineage-specific chimerism analysis after allogeneic stem cell transplantation1,2.

Columns
autoMACS® Columns or Whole Blood Columns.

Overview
StraightFrom® Whole Blood CD4 MicroBeads were developed for rapid automated positive selection of CD4+ cells directly from whole blood or bone marrow. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis.