Miltenyi, 130-090-878, StraightFrom® Whole Blood CD8 MicroBeads, human

Background information
In whole blood the CD8 antigen is expressed on human cytotoxic T cells and on a subset of CD16+ NK cells. The CD8 antigen acts as an accessory molecule in the recognition of MHC class I/peptide complexes by the TCR heterodimer on CD8+ cytotoxic T cells.

Detailed separation procedure
Anticoagulated cell samples of 0.25–15 mL are labeled with StraightFrom® Whole Blood CD8 MicroBeads to be subsequently separated with the autoMACS® Pro Separator, the MultiMACS™ Cell24 Separator Plus, or using the Whole Blood Column Kit.

Downstream applications
StraightFrom® Whole Blood MicroBeads allow the fast isolation of CD8+ cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yield of target cells. StraightFrom Whole Blood MicroBeads in combination with the autoMACS® Pro Separators allow standardization of the cell separation procedure and safe handling of hazardous samples1. The purified CD8+ cells are well-suited for further flow cytometric, functional, or molecular analysis including lineage-specific chimerism analysis after allogeneic stem cell transplantation2,3.

Columns
autoMACS® Columns or Whole Blood Columns.

Overview
StraightFrom® Whole Blood CD8 MicroBeads were developed for rapid positive selection of CD8+ cells directly from whole blood or bone marrow. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis.