Miltenyi, 130-090-879, StraightFrom® Whole Blood CD14 MicroBeads, human

Background information
The CD14 antigen belongs to the LPS receptor complex. It is strongly expressed on most monocytes and macrophages and weakly on neutrophils.

Detailed separation procedure
Anticoagulated cell samples of 0.25–15 mL are labeled with StraightFrom® Whole Blood CD14 MicroBeads to be subsequently separated with the autoMACS® Pro Separator, the MultiMACS™ Cell24 Separator Plus, or using the Whole Blood Column Kit.

Downstream applications
StraightFrom® Whole Blood MicroBeads allow the fast isolation of CD14+ cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yield of target cells. StraightFrom Whole Blood MicroBeads in combination with the autoMACS® Separators allow standardization of the cell separation procedure and safer handling of hazardous samples. The purified CD14+ cells are well-suited for further flow cytometric, functional, or molecular analysis including lineage-specific chimerism analysis after allogeneic stem cell transplantation1,2.

Columns
autoMACS® Columns or Whole Blood Columns.

Overview
StraightFrom® Whole Blood CD14 MicroBeads have been developed for rapid positive selection of CD14+ cells directly from whole blood or bone marrow. No sample preparation is required, no density gradient centrifugation or erythrocyte lysis.