Miltenyi, 130-091-058, StraightFrom® Whole Blood CD15 MicroBeads, human

Background information
StraightFrom® Whole Blood CD15 MicroBeads recognize the carbohydrate structure 3-fucosyl-N-acetyllactosamine (3-FAL), also designated Lewis X. The CD15 antigen is expressed on neutrophils and eosinophils but not on basophils and lymphocytes.

Detailed separation procedure
Anticoagulated cell samples of 0.25–15 mL are labeled with StraightFrom® Whole Blood CD15 MicroBeads to be subsequently separated with the autoMACS® Pro Separator, the MultiMACS™ Cell24 Separator Plus, or using the Whole Blood Column Kit.

Downstream applications
StraightFrom® Whole Blood MicroBeads allow the fast isolation of CD15+ cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yield of target cells. StraightFrom Whole Blood MicroBeads in combination with the autoMACS® Separators allow standardization of the cell separation procedure and safe handling of hazardous samples. The purified CD15+ cells are well-suited for further flow cytometric, functional, or molecular analysis including lineage-specific chimerism analysis after allogeneic stem cell transplantation1,2,3.

Columns
autoMACS® Columns or Whole Blood Columns.

Overview
StraightFrom® Whole Blood CD15 MicroBeads have been developed for rapid positive selection of CD15+ cells directly from whole blood or bone marrow. No sample preparation is required, no density gradient centrifugation or erythrocyte lysis.