Miltenyi, 130-091-086, Blood Dendritic Cell Phenotyping Cocktail Kit, anti-human

Name: Blood Dendritic Cell Phenotyping Cocktail Kit, anti-human
Brand: Miltenyi
SKU: 130-091-086

Size:for 50 tests

Catalog Number: 130-091-086

Pricing

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Category

Soil Test Kits

TagsMACS Flow Cytometry Flow cytometry reagents and kits Phenotyping assays

Description

Background information
BDC Phenotyping Cocktail, anti-human containing:
- CD1c (BDCA-1) Antibody, anti-human, PE (clone: AD5-8E7, isotype: mouse IgG2a)
- CD303 (BDCA-2) Antibody, anti-human, FITC (clone: AC144, isotype: mouse IgG1)
- CD141 (BDCA-3) Antibody, anti-human, APC (clone: AD5-14H12, isotype: mouse IgG1)
- CD20 Antibody, anti-human, PerCP (clone: LT20, isotype: mouse IgG1κ)
- CD14 Antibody, anti-human, PerCP (clone: TÜK4, isotype: mouse IgG2aκ)

BDC Control Cocktail, anti-human containing:
- Isotype Control Antibody, mouse IgG2a, PE (clone: S43.10, isotype: mouse IgG2a)
- Cytokeratin Antibody, anti-human, FITC (clone: CK3-6H5, isotype: mouse IgG1κ)
- Biotin Antibody, APC (clone: Bio3-18E7, isotype: mouse IgG1)
- CD20 Antibody, anti-human, PerCP (clone: LT20, isotype: mouse IgG1κ)
- CD14 Antibody, anti-human, PerCP (clone: TÜK4, isotype: mouse IgG2aκ)

Additionally
- Red Blood Cell Lysis Solution (10×)
- Dead Cell Discriminator
- Discriminator Stop Reagent
- Fix Solution

Instrument details
Flow cytometer equipped with a red (640 nm) and a blue (488 nm) laser.

Applications
Identification and enumeration of DCs and DC subsets in whole blood or peripheral blood mononuclear cells (PBMCs).

Overview
The Blood Dendritic Cell Enumeration Kit was developed for easy identification and enumeration of dendritic cells (DCs) and DC subsets in whole blood or PBMCs by flow cytometry. All reagents required for the analysis are included in the kit.
Applications of the kit include monitoring of peripheral blood DC frequency and number, for example, after hematopoietic stem cell mobilization3, or in the course of pathogenic infections, for example by HIV.4 Furthermore, DC numbers were determined in patients with chronic graft versus host disease5, inflammatory bowel disease6, psoriatic arthritis and rheumatoid arthritis10, under long-term immunosuppression8, lymphoproliferative disease of granular lymphocytes9, or in patients with hepatocellular carcinoma, in order to analyze the immunosuppressive role of IL-10 on circulating dendritic cells7.

Ordering

Notice

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