Miltenyi, 130-091-262, CD4+ Dendritic Cell Isolation Kit, mouse

Background information
CD4+ DCs coexpress CD11c, CD11b, MHC class II, CD40, CD80, CD86 and are negative for CD8 and CD205. They represent the major population of DCs in spleen and comprise only a small population in peripheral lymph nodes (56% and 4% of all CD11c+ DCs, respectively). The isolation of CD4+ DCs is performed in two steps.

Detailed separation procedure
First, DCs are enriched by depletion of T cells, NK cells, B cells, and granulocytes with a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Subsequently, CD4+ DCs are positively selected from the enriched DC fraction with CD4 MicroBeads.

Downstream applications
CD4+ DCs isolated using the CD4+ Dendritic Cell Isolation Kit are suitable to analyze antigen uptake, antigen processing, T cell activation or T cell tolerance induction, cross-priming of cytotoxic T cells, and T cell polarization by CD4+ DCs.

Columns
For the first magnetic separation (depletion): LD or autoMACS® Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.

Overview
The CD4+ Dendritic Cell Isolation Kit is an indirect magnetic labeling system designed to isolate CD4+ DCs from single-cell suspensions of splenocytes.