Miltenyi, 130-091-274, CD294 (CRTH2) MicroBead Kit, human

Name: CD294 (CRTH2) MicroBead Kit, human
Brand: Miltenyi
SKU: 130-091-274

Size:for 1×109 total cells

Catalog Number: 130-091-274

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Category

Human Primary Cells

TagsMACS Cell Separation Cell separation reagents MicroBeads and Isolation Kits T cells

Description

Background information
CD294, also known as CRTH2, is a receptor for prostaglandin D2. CD294 is expressed on T helper type 2 (TH2) cells, a subpopulation of CD4+ T cells, but is not present on TH1 cells. CD294 is highly expressed on peripheral blood basophils and eosinophils. Furthermore, it is present on a small population of CD8+ T cells as well as on a subpopulation of dendritic cells.
For isolation of the CD294+ T helper cell subset, the CD294 (CRTH2) MicroBead Kit can be combined with the CD4+ T Cell Isolation Kit or the CD4 MultiSort Kit. A special protocol is available.

Detailed separation procedure
The CD294+ cells are fluorescently and indirectly magnetically labeled with CD294-PE and Anti-PE MicroBeads.

Columns
For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, D, or autoMACS Columns.

Overview
The CD294 (CRTH2) MicroBead Kit was developed for the positive selection or depletion of CD294+ cells from human PBMCs.

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