Miltenyi, 130-091-329, Large Scale IFN-γ Secretion Assay – Enrichment Kit, human

Background information
The Large Scale IFN-γ Secretion Assay Enrichment Kit is exclusively designed for research use. Nevertheless, it can be used in combination with the manual cell separation systems as well as the autoMACS® Separator, the autoMACS® Pro Separator, and the CliniMACS® Plus Instrument.The MicroBeads are directly conjugated to a secondary IFN-γ-specific antibody (see figure 1). The kit includes Anti-IFN-γ-PE staining antibodies for flow cytometric analysis.

Applications
The Large Scale IFN-γ Secretion Assay - Enrichment Kit allows the concomitant enrichment of viable CD4- and CD8-positive IFN-γ secreting T cells, e.g., for the large-scale generation of antigen-specific cell lines or clones. Tedious subcloning is not necessary and both CD4+ and CD8+ T cell lines may easily be expanded.

Overview
The Large Scale IFN-γ Secretion Assay - Enrichment Kit is suitable for the enrichment of human IFN-γ-secreting, antigen-specific T cells from leukapheresis harvests, PBMCs, or other leukocyte-containing single-cell preparations. Different from the standard Cytokine Secretion Assays, this assay uses a direct strategy for the magnetic labeling of the target cells.