Description
The FASER (Fluorescence Amplification by Sequential Employment of Reagents) Kits – FITC, PE, and APC were developed to increase the fluorescence intensity of cells labeled with virtually any antibody conjugated to FITC (fluorescein-isothiocyanate), PE (R-phycoerythrin) or APC (allophycocyanin). Fluorescence intensity is amplified by the sequential addition of two reagents: The fluorochrome-specific Activator (Reagent 1) and the fluorochrome-conjugated Enhancer (Reagent 2) (see fig. 3). For further enhancement of fluorescence intensity, the sequential addition of reagents can be performed as often as required. The FASER Kits do not affect direct staining with other fluorochrome-conjugated antibodies or MACS® MicroBeads but should not be used in combination with biotin-conjugated antibodies. They are suitable for fresh or formaldehyde-fixed cells in suspension of any type or any species. Analysis is performed by flow cytometry.
Overview
The FASER Kits can be used to amplify weak fluorescence intensity resulting from staining of low-expressed antigens, the use of low-affinity antibodies, or immunomagnetic and fluorochrome-conjugated antibody labeling of cells via the same epitope. Furthermore, increasing the fluorescence intensity simplifies the flow cytometric discrimination of labeled and non-labeled cell fractions. The FASER Kits can also be used in combination with Anti-FITC, Anti-PE or Anti-APC MicroBeads for the enhancement of weak magnetic labeling.
