Miltenyi, 130-092-211, Lineage Cell Depletion Kit, human

Detailed separation procedure
Cells are first labeled with a cocktail of biotin-conjugated antibodies against lineage-specific antigens (CD2, CD3, CD11b, CD14, CD15, CD16, CD19, CD56, CD123, and CD235a (Glycophorin A)). These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads. Lineage-negative stem and progenitor cells are obtained by depletion of the magnetically labeled cells.

Downstream applications

- Flow cytometric characterization of rare stem and progenitor cell subsets
- Evaluation of hematopoietic differentiation potential in vitro using CFU assays (CFC, CFU-GM, BFU-E, and CFU-Mk)
- Evaluation of the CFU-F potential

Columns
MS, LS, XS, or autoMACS® Columns.

Overview
The Lineage Cell Depletion Kit is an indirect magnetic labeling system for the depletion of mature hematopoietic cells such as T cells, B cells, NK cells, dendritic cells, monocytes, granulocytes, erythroid cells, and their committed precursors from bone marrow aspirate, cord blood, or mobilized leukapheresis product. The depletion of lineage-committed cells results in the enrichment of untouched stem and progenitor cells.