Miltenyi, 130-092-662, Basophil Isolation Kit II, human

Name: Basophil Isolation Kit II, human
Brand: Miltenyi
SKU: 130-092-662

Size:for 2×109 total cells

Catalog Number: 130-092-662

Pricing

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Category

Total RNA Isolation Kits

TagsMACS Cell Separation Cell separation reagents MicroBeads and Isolation Kits Granulocytes and myeloid cells

Description

Detailed separation procedure
Non-basophils (i.e. T cells, NK cells, B cells, monocytes, dendritic cells, erythroid cells, platelets, neutrophils, and eosinophils) are indirectly magnetically labeled using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Isolation of highly pure basophils is achieved by depletion of the magnetically labeled cells.

Downstream applications
Isolated basophils have been used for phenotypical characterization3, for histamine, leukotriene, and cytokine1, 4, 5, 6 release assays, for molecular studies1, 2, 5, protein analysis4, 5, and other applications.

Columns
LS, XS, or autoMACS® Columns.

Overview
The Basophil Isolation Kit II is an indirect magnetic labeling system for the negative isolation of basophils from PBMCs.

Ordering

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