Miltenyi, 130-092-924, CD105 MultiSort Kit (PE), mouse

Background information
CD105, also known as Endoglin, is abundantly expressed on angiogenic endothelial cells. In mouse bone marrow, CD105 is also expressed on a subpopulation of Sca-1+ hematopoietic stem cells that displays long-term repopulating activity in lethally irradiated mice (LTR-HSCs)1.

Detailed separation procedure
In the first step, CD105+ cells are positively selected after labeling with the CD105 MultiSort MicroBeads. Subsequently, the MultiSort MicroBeads are enzymatically released from the CD105 antibody. The selected CD105+ cells can be magnetically labeled and sorted according to a second antigen. The kits contains CD105 MultiSort MicroBeads, MultiSort Release Reagent, and the MultiSort Stop Reagent.

Downstream applications
Isolated CD105+Sca-1+ cells can be transplanted into lethally irradiated mice to evaluate the long-term engraftment potential of this cell population.

Columns
MS, LS, or autoMACS® Columns.

Overview
The CD105 MultiSort Kit (PE), mouse has been developed for the isolation of CD105+ cells or cell subsets, including
- vascular endothelial cells from mouse tissue or cell cultures
- long-term repopulating hematopoietic stem cells (LTR-HSCs) from mouse bone marrow when used in combination with the Anti-Sca-1 MicroBead Kit (FITC).