Miltenyi, 130-093-247, CD28 MicroBead Kit, human

Background information
The CD28+ cells are indirectly magnetically labeled with CD28-PE and Anti-PE MicroBeads.
The CD28 antigen is a type I trans‑membrane protein, highly expressed on most CD4+ T cells, many CD8+ T cells, and on malignant plasma cells (myeloma cells). Ligation of CD28 with CD80 (B7-1) or CD86 (B7-2) on target cells provides a critical costimulatory signal for T cell activation. Blockade of CD28-B7 interaction leads to an inhibition of immune responses both in vivo and in vitro.

Downstream applications
The CD28 MicroBead Kit was developed for the isolation of CD28+ cells from PBMCs, e.g., for phenotypical or functional characterization.
CD28+ T cell subsets can be isolated in combination with kits for untouched T cell isolation, e.g., the CD4+ T Cell Isolation Kit or the CD8+ T Cell Isolation Kit.

Columns
For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, D, or autoMACS Columns.

Overview
The CD28 MicroBead Kit was developed for the positive selection or depletion of CD28+ cells from human PBMCs.