Miltenyi, 130-093-476, CD137 MicroBead Kit, human

Background information
CD137-PE recognizes the CD137 (4-1BB) antigen, a 30 kDa glycoprotein of the tumor necrosis factor (TNF) receptor superfamily. CD137 is mainly expressed on activated CD4+ and CD8+ T cells, activated B cells, and on natural killer cells, but can also be found on resting monocytes and dendritic cells. As a costimulatory molecule CD137 is involved in the activation and survival of CD4+ or CD8+ T cells, and NK cells.
Recently, CD137 has been described to be a suitable marker for antigen-specific activation of human CD8+ T cells, as CD137 is not expressed on resting CD8+ T cells and its expression is reliably induced after 24 hours of stimulation.1 CD137+ cells are indirectly magnetically labeled with CD137-PE and Anti-PE MicroBeads. For the isolation of activated antigen-specific CD8+ T cells, the CD137 MicroBead Kit can be combined with the CD8+ T Cell Isolation Kit.

Downstream applications
In a previous study, CD137-expressing T cells were targeted for selective depletion of alloreactive T cells from antileukemic and antitumor donor T cell lines.2 The CD137 MicroBead Kit was developed for positive selection of CD137+ cells for phenotypical and functional characterization of activated CD137+ T cells.

Columns
MS, LS, or autoMACS® Columns.

Overview
The CD137 MicroBead Kit was developed for the positive selection of human CD137+ cells from PBMCs.