Miltenyi, 130-093-477, EPC Analysis Cocktail Kit, anti-human

Name: EPC Analysis Cocktail Kit, anti-human
Brand: Miltenyi
SKU: 130-093-477

Size:for 20 tests

Catalog Number: 130-093-477

Pricing

Send an RFQ.

View RFQ List

Category

Soil Test Kits

TagsMACS Flow Cytometry Flow cytometry reagents and kits Phenotyping assays

Description

Background information
EPC Analysis Cocktail, anti-human containing:
- CD34 Antibody, anti-human, FITC (clone: AC136, isotype: mouse IgG2a)
- CD133/2 Antibody, anti-human, PE (clone: 293C3, isotype: mouse IgG2b)
- CD14 Antibody, anti-human, PerCP (clone: Tük4, isotype: mouse IgG2a)
- CD309 (VEGFR-2) Antibody, anti-human, APC (clone: ES-20E6, isotype: mouse IgG1)

EPC Control Cocktail CD309, anti-human containing:
- CD34 Antibody, anti-human, FITC (clone: AC136, isotype: mouse IgG2a)
- CD133/2 Antibody, anti-human, PE (clone: 293C3, isotype: mouse IgG2b)
- Isotype Control Antibody, mouse IgG1, APC (clone: IS5-21F5)
- CD14 Antibody, anti-human, PerCP (clone: Tük4, isotype: mouse IgG2a)

EPC Control Cocktail CD133, anti-human containing:
- CD34 Antibody, anti-human, FITC (clone: AC136, isotype: mouse IgG2a)
- Isotype Control Antibody, mouse IgG2b, PE (clone: IS6-11E)
- CD14 Antibody, anti-human, PerCP (clone: Tük4, isotype: mouse IgG2a)

Additionally
- EPC Enrichment Cocktail, anti-human
- FcR Blocking Reagent, human
- Red Blood Cell Lysis Solution (25×)
- Propidium Iodide Solution

Instrument details
Flow cytometer equipped with a red (640 nm) and a blue (488 nm) laser.

Applications
Identification and enumeration of CD34+CD309 (VEGFR-2/ KDR)+ and CD133+ endothelial progenitor cells from human peripheral blood, leukapheresis, cord blood, and bone marrow.

Columns
MS or MACSQuant® Columns.

Overview
The EPC Enrichment and Enumeration Kit is designed for the enumeration of circulating EPCs (cEPCs) from peripheral blood, cord blood, or leukapheresis products as well as EPCs from bone marrow. To ensure reliable enumeration results, the EPCs are enriched during the procedure to increase the sensitivity of the flow cytometric analysis.
The kit contains all reagents for:
- red blood cell lysis,
- EPC enrichment,
- blocking of Fc receptor–mediated non-specific labeling of non-target cells,
- enumeration of EPCs based on expression of CD34, CD133, and CD309,
- exclusion of CD14+ cells,
- exclusion of dead cells,
- isotype controls.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.