Miltenyi, 130-093-564, Human FGF-2, premium grade

Biological activity

Background information
Fibroblast growth factor 2 (FGF-2), also termed fibroblast growth factor basic (FGF-b) or basic FGF (bFGF), belongs to the FGF family. It functions as a wide-spectrum mitogenic, angiogenic, and neurotrophic factor and stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal, and endothelial cells. FGF-2 has been implicated in a multitude of physiological and pathological processes, including limb development, angiogenesis, wound healing, and tumor growth.

Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Overview
Recombinant human FGF-2 (FGF-basic or bFGF) is used for the cultivation of undifferentiated embryonic and induced pluripotent stem cells. The basic fibroblast growth factor (FGF) also promotes the growth of a variety of cell types belonging to the mesodermal, ectodermal, and endodermal lineage. Thus, it has implications for diverse processes, such as angiogenesis, tissue repair, neuronal functions or embryogenesis.