Description
Background information
CD146 is a transmembrane glycoprotein belonging to the immunoglobulin superfamily and contains five extracellular immunoglobulin (Ig)-like domains.1,2
CD146 possesses a limited tissue expression distribution, including endothelial cells, smooth muscle cells, pericytes, follicular dendritic cells, melanoma cells, and a subpopulation of activated T lymphocytes.1,3 CD146+ cells isolated from umbilical cord4, lipoaspirate5, dental pulp, or endometrial tissue6, are described to contain cells with clonogenic fibroblastic colony (CFU-F) activity and multilineage differentiation potential, which are often called mesenchymal or marrow stromal cells (MSCs).
CD146 functions as a Ca2+-independent cell adhesion molecule, which is involved in heterophilic cell-cell interactions7, and may be involved in the extravasion and/or homing of activated T cells3. CD146 expression in melanoma is also directly associated with tumor growth and metastasis.7,8
Applications
The CD146 MicroBead Kit can be used for:
- isolation of human mesenchymal stromal cells (MSCs) from lipoaspirate and other tissue sources,
- isolation of endothelial cells and pericytes from human endothelial tissue,
- isolation of human umbilical vein endothelial cells (HUVECs) and human umbilical cord perivascular cells (HUCPVCs)4 from umbilical cord vein.
Columns
LS or XS Columns.
Overview
The CD146 MicroBead Kit was developed for the isolation of CD146+ cells. The kit consists of CD146 MicroBeads and FcR Blocking Reagent. The CD146 MicroBeads recognize the human CD146 antigen, also known as MUC18, MCAM, Mel-CAM, and S-Endo-1.
