Miltenyi, 130-093-634, CD11b (Microglia) MicroBeads, human and mouse

Name: CD11b (Microglia) MicroBeads, human and mouse
Brand: Miltenyi
SKU: 130-093-634

Size:for 1×109 total cells

Catalog Number: 130-093-634

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Category

Human Primary Cells

TagsMACS Cell Separation Cell separation reagents MicroBeads and Isolation Kits Neural cells

Description

Background information
Microglia, often called brain macrophages, are the resident immune effector cells in the central nervous system (CNS). In addition, activated microglia serve as the major antigen-presenting cells in the CNS. They are morphologically, immunophenotypically, and functionally related to cells of the monocyte/macrophage lineage.
This detailed protocol using CD11b (Microglia) MicroBeads allows the fast isolation of primary microglia within 1 hour. The use of the MACS Neural Tissue Dissociation Kit (P) prior to the purification leads to highest yield and viability.
For the isolation of microglia from myelin-containing tissue samples (human tissue or adult mouse or rat), the use of Myelin Removal Beads II is highly recommended.

Downstream applications
CD11b (Microglia) MicroBeads have been used to isolate CD11b+ cells from single-cell suspensions of brain tissue3-6.

Columns
MS, LS, LD, autoMACS® Columns or Multi-24 Column Blocks.

Overview
CD11b (Microglia) MicroBeads have been developed for the positive isolation of primary mouse and human CD11b+ microglia. The purification protocol takes approx. one hour.

Ordering

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