Miltenyi, 130-094-061, Mouse IL-4, research grade

Biological activity

Background information
IL-4 is produced mainly by activated TH2 cells and, to a lesser extent, by TH1 cells. It binds to the IL-4 receptor α (CD124), followed by the dimerization with other receptor chains to generate type 1 and type 2 receptors. IL-4 promotes the proliferation and differentiation of activated B cells and the expression of MHC class II antigens. Mice over expressing IL-4 have elevated levels of IgE and IgG1 and show a deficiency in T cell maturation. CD4+ T cells from knockout mice lacking IL-4 are not able to produce TH2 cytokines after in vitro stimulation. The biological activity of IL-4 is species-specific, i.e. murine IL-4 is inactive on human cells and vice versa.

Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Overview
IL-4 stands for interleukin 4. Mouse IL-4 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.