Miltenyi, 130-094-125, CD4+ Effector Memory T Cell Isolation Kit, human

Name: CD4+ Effector Memory T Cell Isolation Kit, human
Brand: Miltenyi
SKU: 130-094-125

Size:for 1×109 total cells

Catalog Number: 130-094-125

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Category

Human Primary Cells

TagsMACS Cell Separation Cell separation reagents MicroBeads and Isolation Kits T cells

Description

Detailed separation procedure
For magnetic depletion of non-target cells, PBMCs are incubated with a cocktail of biotinylated antibodies against CD45RA, CD8, CD14, CD15, CD16, CD19, CD34, CD36, CD56, CD123, Anti-TCRγ/δ, and CD235a (glycophorin A) as well as APC-conjugated CD197 (CCR7) antibodies. In a second step, all antibody-labeled cells are labeled with MicroBead Cocktail for depletion. The CD4+ Effector Memory T Cell Isolation Kit provides a fast and efficient way to isolate untouched CD4+ effector memory T cells.

Downstream applications
CD4+ effector memory T cells isolated with the CD4+ Effector Memory T Cell Isolation Kit can be used for functional studies, for studies on signal transduction, or for analysis of cytokine expression.

Columns
LD or autoMACS® Columns.

Overview
The Effector Memory CD4+ T Cell Isolation Kit was developed for the isolation of untouched memory T helper cells from human peripheral blood.

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