Miltenyi, 130-094-531, Diamond CD34 Isolation Kit, human

Background information
The CD34 antigen is the most common marker for the isolation of hematopoietic stem and progenitor cells, but is also expressed on some lineage-committed cells. With the Diamond CD34 Isolation Kit, lineage-positive cells are removed in order to obtain pure, primitive CD34+ HSCs.

Detailed separation procedure
The Diamond CD34 MicroBead Kit includes a lineage depletion followed by a CD34 isolation. In the first step, cells are labeled with a cocktail of biotin-conjugated antibodies against lineage-specific antigens. These cells are subsequently labeled with Anti-Biotin MicroBeads. Lineage-negative stem and progenitor cells are obtained by depletion of the magnetically labeled cells. Then CD34+ cells are isolated from the lineage-negative stem and progenitor cells using CD34 MicroBeads. The resulting population obtained are CD34+Lin– stem cells.

Downstream applications
Purified CD34+Lin- stem cells can be used for many applications, including:
- gene or miRNA expression profiling
- characterization of undifferentiated stem cells and their developmental potential
- stimulation of proliferation and maturation by cytokines
- HSC-CFU assays
- adoptive transfer and hematopoietic reconstitution

Columns
For positive selection: MS or autoMACS® Columns. For depletion: LD or autoMACS Columns.

Overview
The Diamond CD34 Isolation Kit has been specifically developed for the isolation of highly pure Lin–CD34+ hematopoietic stem cells from peripheral blood1, bone marrow2,3 or cord blood.