Miltenyi, 130-094-532, Mitochondria Isolation Kit, human

The Mitochondria MidiMACS Starting Kit, human includes:
- 1 Mitochondria Isolation Kit, human
- 1 MidiMACS Separator
- 1 MACS MultiStand
The Mitochondria QuadroMACS Starting Kit, human includes:
- 1 Mitochondria Isolation Kit, human
- 1 QuadroMACS Separator
- 1 MACS MultiStand

Detailed procedure
After cell lysis, mitochondria are magnetically labeled with Anti-TOM22 MicroBeads, human, which bind to the translocase of the outer mitochondrial membrane 22 protein (TOM22). The sample is loaded onto an LS Column placed in a MidiMACS or QuadroMACS Separator. After washing, only magnetically labeled mitochondria are retained on the column. The column is removed from the separator and functional human mitochondria are eluted from the column.

Downstream applications
After isolation mitochondria can be further subjected to Western blot analysis1,6 or downstream functional assays, including measurement of O2, membrane potential, and ATP4-5,7, or respiratory control4. Growing evidence also suggests that isolated mitochondria are well suited for mitochondrial RNA expression profiling studies2,3.

Overview
The Mitochondria Isolation Kit, human facilitates the isolation of functional and viable mitochondria from human cells or tissue. The isolation protocol is based on the renowned MACS Technology, which enables fast isolation of high purity and high yield mitochondria.