Miltenyi, 130-094-773, StraightFrom® Whole Blood CD33 MicroBeads, human

Background information
The CD33 antigen is prominently expressed on myeloid progenitor cells and monocytes, whereas low-level expression is observed on peripheral granulocytes, dendritic cells, and tissue macrophages. It is not expressed on lymphocytes, platelets, erythrocytes, and primitive hematopoietic stem cells.

Detailed separation procedure
Anticoagulated cell samples of 0.25–15 mL are labeled with StraightFrom® Whole Blood CD33 MicroBeads to be subsequently separated with the autoMACS® Pro Separator, the MultiMACS™ Cell24 Separator Plus, or using the Whole Blood Column Kit.

Downstream applications
StraightFrom® Whole Blood MicroBeads allow the fast isolation of CD33+ cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yield of target cells. StraightFrom Whole Blood MicroBeads in combination with the autoMACS® Separators allow standardization of the cell separation procedure and safe handling of hazardous samples. The purified CD33+ cells are well-suited for further flow cytometric, functional, or molecular analysis including lineage-specific chimerism analysis after allogeneic stem cell transplantation.

Columns
autoMACS® Columns or Whole Blood Columns.

Overview
StraightFrom® Whole Blood CD33 MicroBeads have been developed for rapid positive selection of CD33+ cells directly from whole blood or bone marrow. No sample preparation is required, no density gradient centrifugation or erythrocyte lysis.