Miltenyi, 130-094-913, Diamond CD133 Isolation Kit, human

Detailed separation procedure
The Diamond CD133 MicroBead Kit includes a lineage depletion followed by a CD133 isolation. In the first step, cells are labeled with a cocktail of biotin-conjugated antibodies against lineage-specific antigens. These cells are subsequently labeled with Anti-Biotin MicroBeads. Lineage-negative stem and progenitor cells are obtained by depletion of the magnetically labeled cells. Then CD133+ cells are isolated from the lineage-negative stem and progenitor cells using CD133 MicroBeads. The resulting population obtained are Lin-CD133+ stem cells.
Diamond CD133 MicroBeads recognize the epitope CD133/1. For control staining of separated cells, the application of CD133/2 (293C3) antibody is recommended.

Downstream applications
Lin-CD133+stem cells can be used for many applications, including:
- gene or miRNA expression profiling
- characterization of undifferentiated stem cells and their developmental potential
- stimulation of proliferation and maturation by cytokines
- HSC-CFU assays
- adoptive transfer and hematopoietic reconstitution studies

Columns
For the first magnetic separation (depletion): LD or autoMACS® Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.

Overview
The Diamond CD133 Isolation Kit was specifically developed for the isolation of highly pure Lin–CD133+ stem cells from peripheral blood, cord blood, bone marrow, and leukapheresis product by sequential sorting.