Miltenyi, 130-094-945, CD127 MicroBead Kit, human

Background information
CD127, the α-chain of the IL-7 receptor, is a type I membrane glycoprotein. Signaling of IL-7 through the IL-7R requires both IL-7Rα and the common cytokine gamma chain (γc).1 CD127 can be identified on immature B cells through the early pre-B stage, on thymocytes, and on most mature T cells with transient down-regulation upon activation.2,3 On regulatory T cells CD127 is absent4 and its expression is inversely correlated with FoxP3 expression and suppressive function.5,6 CD127 is also used by thymic stromal derived lymphopoietin (TSLP) as part of a complex.1
Human regulatory T cells can be isolated, for example, by using the CD4+CD25+CD127dim/– Regulatory T Cell Isolation Kit, human.

Columns
For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, D, or autoMACS Columns.

Overview
The CD127 MicroBead Kit has been developed for the positive selection or depletion of CD127+ cells from human PBMCs.