Miltenyi, 130-095-130, Pan T Cell Isolation Kit II, mouse

Background information
The kit is based on a cocktail of biotin-conjugated antibodies against CD11b, CD11c, CD19, CD45R (B220), CD49b (DX5), CD105, Anti-MHC-class II, and Ter-119. The isolation of T cells is achieved by depletion of magnetically labeled cells.

Downstream applications
Untouched T cells are used for functional studies in which cross-linking of surface molecules should be excluded. Isolated T cells can also be further magnetically separated into T cell subsets.
The Pan T Cell Isolation Kit has been used for adoptive transfer experiments1,2, T cell proliferation assays3,4, coculture with dendritic cells5, and cytokine analysis6 experiments.

Columns
LS or autoMACS® Columns.

Overview
The Pan T Cell Isolation Kit II was developed for the isolation of untouched mouse T cells from single-cell suspensions from spleen or lymph nodes.