Miltenyi, 130-095-873, Regulatory B Cell Isolation Kit, mouse

Name: Regulatory B Cell Isolation Kit, mouse
Brand: Miltenyi
SKU: 130-095-873

Size:for 2×109 total cells

Catalog Number: 130-095-873

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Category

Total RNA Isolation Kits

TagsMACS Cell Separation Cell separation reagents MicroBeads and Isolation Kits B cells

Description

Detailed procedure
The isolation of regulatory B cells is performed in three steps. First, B cells are pre-enriched by depletion of non-B cells. Second, the pre-enriched B cells are stimulated for 5 hours in culture with, e.g., LPS, PMA, and ionomycin to induce IL-10 secretion. Third, the viable IL-10–producing cells are specifically isolated by using the Cytokine Secretion Assay technology.

Downstream applications
Isolated viable IL-10 secreting regulatory B cells can be used for phenotyping and functional analysis.

Columns
For the first magnetic separation (depletion): LD or autoMACS® Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.

Overview
The Regulatory B Cell Isolation Kit was developed for the isolation of viable IL-10-producing regulatory B cells from single-cell suspensions of lymphoid organs.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
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