Description
Background information
The quality of starting material is important for any experiment, and a perfect single-cell suspension is a prerequisite for many downstream applications. Glioblastoma, neuroblastoma, medulloblastoma, xenograft brain tumor samples can be effectively dissociated into single-cell suspensions using the Brain Tumor Dissociation Kit (P) and a gentleMACS™ Dissociator.
The Brain Tumor Dissociation Kit (P) is based on papain. The antigens GLAST (ACSA-1) and PSA-NCAM used to identify some neural populations are sensitive to papain. Immune cell epitopes are also sensitive to papain. If preservation of these antigen epitopes is required for downstream applications, it is more suitable to use in this case the Tumor Dissociation Kit, mouse (# 130-096-730) or Tumor Dissociation Kit, human (# 130-095-929) depending on the species from where the tumor originated.
Overview
The Brain Tumor Dissociation Kit (P) has been developed specifically for the gentle and effective tissue dissociation of human and mouse brain tumors, such as glioblastoma multiforme or medulloblastoma. Since tumor tissue can be sticky, it is recommended to use the kit in combination with the gentleMACS™ Dissociator or the gentleMACS Octo Dissociator. In less than an hour, the tissue dissociation procedure efficiently yields high numbers of viable cells. Dissociated cells are immediately ready for further applications, such as cell culture, flow cytometry, molecular applications, or for cell isolation using MACS® Technology.
