Miltenyi, 130-096-207, MSC Suppression Inspector, human

Background information
MSCs are fibroblast-like plastic-adherent cells that can be isolated from a variety of tissues, such as bone marrow or adipose tissue. During the last few years the attention of scientists was redirected away from the multipotentiality of MSCs towards their possibility for immunomodulation. It was observed that bone marrow derived MSCs suppress T-cell proliferation1,2. This function of MSCs can be analyzed using the MSC Suppression Inspector which contains an optimal T cell stimulation reagent for an MSC suppression assay.

Detailed procedure
The MSC Suppression Inspector consists of Anti-Biotin MACSiBead Particles that are pre-loaded with biotinylated CD2, CD3, and CD28 antibodies. MSCs are co-cultured with CD4+CD25– or CD4+ responder T cells (Tresp) at different ratios in the presence of a polyclonal stimulus, in this case the MSC Suppression Inspector. Tresp cells alone show a proliferative response. Co-culture of MSCs with Tresp cells results in reduced proliferation of Tresp cells. Cell proliferation is determined by 3H-thymidine incorporation or detected by carboxyfluorescein succinimidyl ester (CFSE) staining.

Overview
The MSC Suppression Inspector was developed for the functional characterization of human mesenchymal stem cells, also known as mesenchymal stromal cells (MSCs) by in vitro suppression assays.