Miltenyi, 130-096-354, CD115 MicroBead Kit, mouse

Background information
CD115 is expressed on monocytes, macrophages, osteoclasts, as well as on common dendritic cell precursors and macrophage/dendritic cell precursors. CD115 has been proposed as a marker for the standardized identification and analysis of mouse monocytes.1

Detailed separation procedure
In order to avoid non-specific binding of the antibody, the single-cell suspension is pre-incubated with FcR Blocking Reagent. Target cells are then labeled with a biotinylated CD115 antibody. Subsequently, CD115+ cells are magnetically labeled with Anti-Biotin MicroBeads. After the separation over a MACS Column, the CD115+ cells are eluted as the positive fraction.

Downstream applications
Isolated mouse CD115+ cells, e.g. from bone marrow, can be used for in vitro analysis, differentiation studies, co-culture, or adoptive transfer experiments.

Columns
MS, LS, or autoMACS® Columns.

Overview
The CD115 MicroBead Kit has been developed for positive selection of CD115+ mouse cells from lymphoid tissues.